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rhil4  (R&D Systems)


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    Structured Review

    R&D Systems rhil4
    Rhil4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhil4/Recombinant+Human+IL-4+Protein/pm39229274-172-34-35
    Average 96 stars, based on 1029 article reviews
    rhil4 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Development and characterization of dendritic cell internalization and activation assays contributing to the immunogenicity risk evaluation of biotherapeutics
    Article Snippet: .. CD14+ monocytes were differentiated into monocyte derived DCs (moDCs), by culturing within a DC medium (sterile filtered CellGenix GMP DC medium, with GlutaMAX, non-essential amino acids, sodium pyruvate and Penicillin-Streptomycin) supplemented with 5 ng/mL rhIL4 (R&D systems, #204-IL) and 50 ng/mL rhGM-CSF (R&D system, #215GM-500) for 5 days at 37°C and 5% CO2 ambient on ultra-low attachment culture dishes (0.3x10 6 cells/ml, Corning, #354407). ..

    Article Title: Development and characterization of dendritic cell internalization and activation assays contributing to the immunogenicity risk evaluation of biotherapeutics.
    Article Snippet: .. CD14+ monocytes were differentiated into monocyte derived DCs (moDCs), by culturing within a DC medium (sterile filtered CellGenix GMP DCmedium, with GlutaMAX, non-essential amino acids, sodium pyruvate and Penicillin-Streptomycin) supplemented frontiersin.org with 5 ng/mL rhIL4 (R&D systems, #204-IL) and 50 ng/mL rhGMCSF (R&D system, #215GM-500) for 5 days at 37°C and 5% CO2 ambient on ultra-low attachment culture dishes (0.3x106 cells/ml, Corning, #354407). ..

    Article Title: Internalization of therapeutic antibodies into dendritic cells as a risk factor for immunogenicity
    Article Snippet: .. CD14+ monocytes were differentiated into monocyte derived DCs (moDCs), by culturing within a DC medium (sterile filtered CellGenix GMP DC medium, with GlutaMAX, non-essential amino acids, sodium pyruvate and Penicillin-Streptomycin) supplemented with 5 ng/mL rhIL4 (R&D systems, #204-IL) and 50 ng/mL rhGM-CSF (R&D system, #215GM-500) for 5 days at 37°C and 5% CO 2 ambient on ultra-low attachment culture dishes (0.3x10 6 cells/ml, Corning, #354407). ..

    Sterility:

    Article Title: Development and characterization of dendritic cell internalization and activation assays contributing to the immunogenicity risk evaluation of biotherapeutics
    Article Snippet: .. CD14+ monocytes were differentiated into monocyte derived DCs (moDCs), by culturing within a DC medium (sterile filtered CellGenix GMP DC medium, with GlutaMAX, non-essential amino acids, sodium pyruvate and Penicillin-Streptomycin) supplemented with 5 ng/mL rhIL4 (R&D systems, #204-IL) and 50 ng/mL rhGM-CSF (R&D system, #215GM-500) for 5 days at 37°C and 5% CO2 ambient on ultra-low attachment culture dishes (0.3x10 6 cells/ml, Corning, #354407). ..

    Article Title: Development and characterization of dendritic cell internalization and activation assays contributing to the immunogenicity risk evaluation of biotherapeutics.
    Article Snippet: .. CD14+ monocytes were differentiated into monocyte derived DCs (moDCs), by culturing within a DC medium (sterile filtered CellGenix GMP DCmedium, with GlutaMAX, non-essential amino acids, sodium pyruvate and Penicillin-Streptomycin) supplemented frontiersin.org with 5 ng/mL rhIL4 (R&D systems, #204-IL) and 50 ng/mL rhGMCSF (R&D system, #215GM-500) for 5 days at 37°C and 5% CO2 ambient on ultra-low attachment culture dishes (0.3x106 cells/ml, Corning, #354407). ..

    Article Title: Internalization of therapeutic antibodies into dendritic cells as a risk factor for immunogenicity
    Article Snippet: .. CD14+ monocytes were differentiated into monocyte derived DCs (moDCs), by culturing within a DC medium (sterile filtered CellGenix GMP DC medium, with GlutaMAX, non-essential amino acids, sodium pyruvate and Penicillin-Streptomycin) supplemented with 5 ng/mL rhIL4 (R&D systems, #204-IL) and 50 ng/mL rhGM-CSF (R&D system, #215GM-500) for 5 days at 37°C and 5% CO 2 ambient on ultra-low attachment culture dishes (0.3x10 6 cells/ml, Corning, #354407). ..

    Cell Culture:

    Article Title: Human monocyte-derived dendritic cells as an in vitro alternative model cell to evaluate the immunotoxicity of 2, 4-Dinitrochlorobenzene.
    Article Snippet: Improvements in science and technology have led to the increasing threats of new chemicals to the public health.. It is crucial to evaluate the toxicity, especially immunotoxicology.. Dendritic cells (DCs) are believed to be more favorable choices in immunotoxicity evaluations.

    Article Title: Identification of Immunogenic MHC Class II Human HER3 Peptides that Mediate Anti-HER3 CD4 + Th1 Responses and Potential Use as a Cancer Vaccine.
    Article Snippet: All research involving human subjects was performed in accordance with the Declaration of Helsinki and was approved by the IRB at Advarra. .. Monocytes were differentiated into dendritic cells (DC) through the addition of recombinant human (rh)GM-CSF (50 ng/mL; cat. #215-GM-050, R&D Systems) and rhIL4 (10 ng/mL; cat. #204-IL-050, R&D Systems) and cultured in macrophage serumfree media (cat. #12065074; Thermo Fisher Scientific) for 24 hours at 37 C and 5% CO2. ..

    Article Title: Identification of Immunogenic MHC Class II Human HER3 Peptides that Mediate Anti-HER3 CD4+ Th1 Responses and Potential Use as a Cancer Vaccine
    Article Snippet: All research involving human subjects was performed in accordance with the Declaration of Helsinki and was approved by the IRB at Advarra. .. Monocytes were differentiated into dendritic cells (DC) through the addition of recombinant human (rh)GM-CSF (50 ng/mL; cat. #215-GM-050, R&D Systems) and rhIL4 (10 ng/mL; cat. #204-IL-050, R&D Systems) and cultured in macrophage serumfree media (cat. #12065074; Thermo Fisher Scientific) for 24 hours at 37 C and 5% CO2. ..

    Article Title: Immunogenic Cell Death and Immunomodulatory Effects of Cabozantinib
    Article Snippet: .. Monocytes (CD14 + ) were purified from PBMCs after Ficoll–Hypaque gradient (1,077 g/ml; Pharmacia LKB) using the Human CD14-Positive Selection Kit (StemCell Technologies, Vancouver, Canada) and cultured (5 × 10 5 cells/ml) in Roswell Park Memorial Institute (RPMI)-1640 (Sigma-Aldrich) supplemented with 2 mmol/L L-glutamine, penicillin of 100 U/mL, streptomycin of 100 μg/ml (Sigma-Aldrich), with 5% heat-inactivated FCS (Merck KGaA). rhGM-CSF (50 ng/ml) (R&D Systems, Minneapolis, MN, USA) and 2,000 U/ml rhIL4 (R&D Systems) were added at day 0 and 2. .. Immature DCs (iDCs) were collected on day 5 and matured with cytokine cocktail [rhIL1β, IL6, TNFα (10 ng/ml) all purchased from R&D Systems and PGE 2 (1 μg/ml) purchased from Sigma-Aldrich)] for 16 h. Cabozantinib (2.5 μg/ml) was added at day 4 during DCs differentiation.

    Recombinant:

    Article Title: Identification of Immunogenic MHC Class II Human HER3 Peptides that Mediate Anti-HER3 CD4 + Th1 Responses and Potential Use as a Cancer Vaccine.
    Article Snippet: All research involving human subjects was performed in accordance with the Declaration of Helsinki and was approved by the IRB at Advarra. .. Monocytes were differentiated into dendritic cells (DC) through the addition of recombinant human (rh)GM-CSF (50 ng/mL; cat. #215-GM-050, R&D Systems) and rhIL4 (10 ng/mL; cat. #204-IL-050, R&D Systems) and cultured in macrophage serumfree media (cat. #12065074; Thermo Fisher Scientific) for 24 hours at 37 C and 5% CO2. ..

    Article Title: Identification of Immunogenic MHC Class II Human HER3 Peptides that Mediate Anti-HER3 CD4+ Th1 Responses and Potential Use as a Cancer Vaccine
    Article Snippet: All research involving human subjects was performed in accordance with the Declaration of Helsinki and was approved by the IRB at Advarra. .. Monocytes were differentiated into dendritic cells (DC) through the addition of recombinant human (rh)GM-CSF (50 ng/mL; cat. #215-GM-050, R&D Systems) and rhIL4 (10 ng/mL; cat. #204-IL-050, R&D Systems) and cultured in macrophage serumfree media (cat. #12065074; Thermo Fisher Scientific) for 24 hours at 37 C and 5% CO2. ..

    Purification:

    Article Title: Immunogenic Cell Death and Immunomodulatory Effects of Cabozantinib
    Article Snippet: .. Monocytes (CD14 + ) were purified from PBMCs after Ficoll–Hypaque gradient (1,077 g/ml; Pharmacia LKB) using the Human CD14-Positive Selection Kit (StemCell Technologies, Vancouver, Canada) and cultured (5 × 10 5 cells/ml) in Roswell Park Memorial Institute (RPMI)-1640 (Sigma-Aldrich) supplemented with 2 mmol/L L-glutamine, penicillin of 100 U/mL, streptomycin of 100 μg/ml (Sigma-Aldrich), with 5% heat-inactivated FCS (Merck KGaA). rhGM-CSF (50 ng/ml) (R&D Systems, Minneapolis, MN, USA) and 2,000 U/ml rhIL4 (R&D Systems) were added at day 0 and 2. .. Immature DCs (iDCs) were collected on day 5 and matured with cytokine cocktail [rhIL1β, IL6, TNFα (10 ng/ml) all purchased from R&D Systems and PGE 2 (1 μg/ml) purchased from Sigma-Aldrich)] for 16 h. Cabozantinib (2.5 μg/ml) was added at day 4 during DCs differentiation.

    Selection:

    Article Title: Immunogenic Cell Death and Immunomodulatory Effects of Cabozantinib
    Article Snippet: .. Monocytes (CD14 + ) were purified from PBMCs after Ficoll–Hypaque gradient (1,077 g/ml; Pharmacia LKB) using the Human CD14-Positive Selection Kit (StemCell Technologies, Vancouver, Canada) and cultured (5 × 10 5 cells/ml) in Roswell Park Memorial Institute (RPMI)-1640 (Sigma-Aldrich) supplemented with 2 mmol/L L-glutamine, penicillin of 100 U/mL, streptomycin of 100 μg/ml (Sigma-Aldrich), with 5% heat-inactivated FCS (Merck KGaA). rhGM-CSF (50 ng/ml) (R&D Systems, Minneapolis, MN, USA) and 2,000 U/ml rhIL4 (R&D Systems) were added at day 0 and 2. .. Immature DCs (iDCs) were collected on day 5 and matured with cytokine cocktail [rhIL1β, IL6, TNFα (10 ng/ml) all purchased from R&D Systems and PGE 2 (1 μg/ml) purchased from Sigma-Aldrich)] for 16 h. Cabozantinib (2.5 μg/ml) was added at day 4 during DCs differentiation.



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